Coding
RebC

Part:BBa_K2912002:Design

Designed by: Lingling Liao   Group: iGEM19_SZU-China   (2019-09-08)


RebC may be involved in the modification process of forming the R bodies


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The third ORF, rebC, extends from base 1393 through 1563. The initiation codon for rebC is preceded by a possible RBS GGGA (bases 1378 to 1381). The probable -10 promoter sequences, TATTAT (bases 1363 to 1368). The rebC gene utilizes the TAG stop codon, and transcriptional termination appears to be rho dependent.


Source

This part is one of the DNA sequences for the R body locus (reb) from Caedibacter taeniospiralis and the sequence is from gene bank.

References

Heruth D P , Pond F R , Dilts J A , et al. Characterization of genetic determinants for R body synthesis and assembly in Caedibacter taeniospiralis 47 and 116.[J]. Journal of Bacteriology, 1994, 176(12):3559-3567.